Nucleosides & nucleotides

Structurally Altered Substrates for DNA Topoisomerase. I. Effects of Inclusion of a Single 3′-Deoxynucleotide Within the Scissile Strand†

T Arslan, AT Abraham, SM Hecht

文献索引：Arslan, Tuncer; Abraham, Anil T.; Hecht, Sidney M. Nucleosides and Nucleotides, 1998 , vol. 17, # 1-3 p. 515 - 530

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被引用次数: 25

摘要

Abstract A partial DNA duplex containing a high efficiency topoisomerase I cleavage site was substituted singly at each of three sites with 3′-deoxyadenosine. Depending on the site of substitution, the facility of the topoisomerase I-mediated cleavage or ligation reactions was altered. Inclusion of the modified nucleoside at the 5′-end of the acceptor oligonucleotide diminished the rate of religation following substrate cleavage by the ...