Assessment of a putative proton relay in Arabidopsis cinnamyl alcohol dehydrogenase catalysis

C Lee, DL Bedgar, LB Davin, NG Lewis

文献索引：Lee, Choonseok; Bedgar, Diana L.; Davin, Laurence B.; Lewis, Norman G. Organic and Biomolecular Chemistry, 2013 , vol. 11, # 7 p. 1127 - 1134

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被引用次数: 2

摘要

Extended proton relay systems have been proposed for various alcohol dehydrogenases, including the Arabidopsis thaliana cinnamyl alcohol dehydrogenases (AtCADs). Following a previous structural biology investigation of AtCAD5, the potential roles of three amino acid residues in a putative proton relay system, namely Thr49, His52 and Asp57, in AtCAD5, were investigated herein. Using site-directed mutagenesis, kinetic and isothermal titration ...