Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual “glycolate” chain extension unit in ansamitocin biosynthesis
…, SJ Moss, L Bai, Y Kato, S Toelzer, TW Yu…
文献索引：Carroll, Brian J.; Moss, Steven J.; Bai, Linquan; Kato, Yasuo; Toelzer, Sabine; Yu, Tin-Wein; Floss, Heinz G. Journal of the American Chemical Society, 2002 , vol. 124, # 16 p. 4176 - 4177
The unusual “glycolate” extender unit at C-9/C-10 of ansamitocin is not derived from 2- hydroxymalonyl-CoA or 2-methoxymalonyl-CoA, as demonstrated by feeding experiments with the corresponding 1-13C-labeled N-acetylcysteamine thioesters but is formed from an acyl carrier protein (ACP)-bound substrate, possibly 2-methoxymalonyl-ACP, elaborated by enzymes encoded by a subcluster of five genes, asm12-17, from the ansamitocin ...
