Screening for new hydroxynitrilases from plants

…, T Ueatrongchit, A H-KITTIKUN, T Ohmiya

文献索引：Asano, Yasuhisa; Tamura, Ken'ichirou; Doi, Nobutaka; Ueatrongchit, Techawaree; H-Kittikun, Aran; Ohmiya, Tohru Bioscience, Biotechnology and Biochemistry, 2005 , vol. 69, # 12 p. 2349 - 2357

全文：HTML全文

被引用次数: 57

摘要

We established a simple HPLC method to determine the activity and stereochemistry of the chiral mandelonitrile synthesized from benzaldehyde and cyanide, and applied it to screen for hydroxynitrile lyase (HNL) activity of plant origin. A total of 163 species of plants among 74 families were examined for (R)-and (S)-HNL activities using the method. We discovered that homogenate of leaves of Baliospermum montanum shows (S)-HNL activity, while ...