Optically trapping confocal Raman microscopy of individual lipid vesicles: kinetics of phospholipase A2-catalyzed hydrolysis of phospholipids in the membrane bilayer
DP Cherney, GA Myers, RA Horton…
文献索引：Cherney, Daniel P.; Myers, Grant A.; Horton, Robert A.; Harris, Joel M. Analytical Chemistry, 2006 , vol. 78, # 19 p. 6928 - 6935
Phospholipase A2 (PLA2)-catalyzed hydrolysis at the sn-2 position of 1, 2-dimyristoyl-sn- glycero-3-phosphocholine in optically trapped liposomes is monitored in situ using confocal Raman microscopy. Individual optically trapped liposomes (0.6 μm in diameter) are exposed to PLA2 isolated from cobra (Naja naja naja) venom at varying enzyme concentrations. The relative Raman scattering intensities of CC stretching vibrations from the trans and gauche ...
