Optimization of non-natural nucleotides for selective incorporation opposite damaged DNA

D Vineyard, X Zhang, A Donnelly, I Lee…

文献索引：Vineyard, Diana; Zhang, Xuemei; Donnelly, Alison; Lee, Irene; Berdis, Anthony J. Organic and Biomolecular Chemistry, 2007 , vol. 5, # 22 p. 3623 - 3630

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被引用次数: 21

摘要

The promutagenic process known as translesion DNA synthesis reflects the ability of a DNA polymerase to misinsert a nucleotide opposite a damaged DNA template. To study the underlying mechanism of nucleotide selection during this process, we quantified the incorporation of various non-natural nucleotide analogs opposite an abasic site, a non- templating DNA lesion. Our kinetic studies using the bacteriophage T4 DNA polymerase ...