Biochimie

Aminolytic reaction catalyzed by D-stereospecific amidohydrolases from Streptomyces spp

J Arima, H Ito, T Hatanaka, N Mori

文献索引：Arima, Jiro; Ito, Hitomi; Hatanaka, Tadashi; Mori, Nobuhiro Biochimie, 2011 , vol. 93, # 9 p. 1460 - 1469

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被引用次数: 4

摘要

From investigation of 2000 soil isolates, we identified two serine-type amidohydrolases that can hydrolyze d-aminoacyl derivatives from the culture supernatant of Streptomyces species 82F2 and 83D12. The enzymes, redesignated as 82F2-DAP and 83D12-DAP, were purified for homogeneity and characterized. Each enzyme had molecular mass of approximately 40 kDa, and each showed moderate stability with respect to temperature and pH. Among ...