Substrate specificity for the epoxidation of terpenoids and active site topology of house fly cytochrome P450 6A1

…, PH Evans, WS Bowers, R Feyereisen

文献索引：Andersen, John F.; Walding, Jennifer K.; Evans, Philip H.; Bowers, William S.; Feyereisen, Rene Chemical Research in Toxicology, 1997 , vol. 10, # 2 p. 156 - 164

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被引用次数: 44

摘要

Heterologous expression in Escherichia coli, purification, and reconstitution of house fly P450 6A1 and NADPH-cytochrome P450 reductase were used to study the metabolism of terpenoids. In addition to the epoxidation of cyclodiene insecticides demonstrated previously [Andersen et al.(1994) Biochemistry 33, 2171-2177], this cytochrome P450 was shown to epoxidize a variety of terpenoids such as farnesyl, geranyl, and neryl methyl esters, ...