A Pseudomonas sp. alcohol dehydrogenase with broad substrate specificity and unusual stereospecificity for organic synthesis

CW Bradshaw, H Fu, GJ Shen…

文献索引：Bradshaw, Curt W.; Fu, Hong; Shen, Gwo-Jenn; Wong, Chi-Huey Journal of Organic Chemistry, 1992 , vol. 57, # 5 p. 1526 - 1532

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被引用次数: 148

摘要

A new alcohol dehydrogenase from Pseudomonas sp. strain PED has been isolated and characterized. The enzyme exhibits a broad substrate specificity, accepting aromatic, cyclic, and aliphatic compounds as substrates. The K, values were determined as 525 HM for NAD and 75 p M for 2-propanol with a specific activity of 36 U/mg. The kinetic mechanism is ordered bi-bi with the cofactor binding first and releasing last. The enzyme transfers the ...