Chemical Communications

A simple photo-affinity labeling protocol

H Li, Y Liu, K Fang, K Nakanishi

文献索引：Li, Hong-Yu; Liu, Ying; Fang, Kan; Nakanishi, Koji Chemical Communications, 1999 , # 4 p. 365 - 366

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被引用次数: 10

摘要

After photo-crosslinking and proteolysis of a photoaffinity labeled ligand to its receptor, the ester group incorporated in the ligand is cleaved by an amine with specific functionality, which displaces the bulk of the ligand from the peptide fragments, and facilitates subsequent tandem mass spectrometric sequencing.