Synthesis and application of a novel, crystalline phosphoramidite monomer with thiol terminus, suitable for the synthesis of DNA conjugates

…, Z Schmél, Z Kele, B Penke, L Kovács

文献索引：Kupihar, Zoltan; Schmel, Zoltan; Kele, Zoltan; Penke, Botond; Kovacs, Lajos Bioorganic and Medicinal Chemistry, 2001 , vol. 9, # 5 p. 1241 - 1247

全文：HTML全文

被引用次数: 14

摘要

A new, crystalline 5′-thiol modifier phosphoramidite monomer (3), suitable for DNA synthesis, has been prepared. This monomer has been built into an oligonucleotide using the standard protocol. After cleavage, purification and removal of the trityl group with Ag+, a free 5′-thiol terminal oligonucleotide (15) has been obtained which was subsequently coupled to a cysteine derivative via a disulfide bridge to afford conjugate 16.