... Biochemical Pharmacology. ... The homogenate was centrifuged at 1000 × g for 5 min at 4 °C, and the supernatant layer was then centrifuged at 36,000 × g for 20 min at 4 °C. The resulting pellet was then suspended in 4 vol (original wet weight) of ice ... Mol Pharmacol, 34 (1988), pp. ...