Abstract Thiamine diphosphate (ThDP)-dependent enzymes like benzaldehyde lyase from Pseudomonas fluorescens (BAL) and benzoylformate decarboxylase from Pseudomonas putida (BFD) are versatile biocatalysts for the C [BOND] C bond ligation of aldehydes to form enantiomerically pure 2-hydroxy ketones. However, the large-scale application of this enzyme class is often restricted by the required external addition of the expensive cofactor ...
