Abstract β-(1, 2, 4-triazol-1-yl) Alanine, an important metabolite of the triazole-based fungicide Myclobutanil, was shown to be derived from O-acetyl-l-serine and 1, 2, 4-triazole by cysteine synthase in higher plants. Some properties of this enzyme in the biosynthesis of β-(1, 2, 4-triazol-1-yl) alanine are described.