In vitro synthesis of 1, N6??etheno??2′??deoxyadenosine and 1, N2??etheno??2′??deoxyguanosine by 2, 4??dinitrophenol and 1, 3??dinitropyrene in presence of a …
Abstract The formation of covalent nitro-PAH DNA adducts and nitro-PAH mediated oxidative lesions are two possible mechanisms for the initiation of nitro-PAH carcinogenesis. Sixty-minute incubation of 1, 3-dinitropyrene (100 μM) or 1, 4-dinitrophenol (100 μM) with a mixture of 150 μM NADH, 0.5 units of E. coli nitroreductase, 100 μM linoleic acid, 0.5 mM ferrous iron, and 100 μM 2′-deoxyadenosine (2′-dA) or 100 μM 2′-deoxyguanosine (2 ...
