Biochemistry

Characterization of unstable products of flavin-and pterin-dependent enzymes by continuous-flow mass spectrometry

KM Roberts, JR Tormos, PF Fitzpatrick

文献索引：Roberts, Kenneth M.; Tormos, Jose R.; Fitzpatrick, Paul F. Biochemistry, 2014 ,  vol. 53,  # 16  p. 2672 - 2679

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被引用次数: 5

摘要

Continuous-flow mass spectrometry (CFMS) was used to monitor the products formed during the initial 0.25–20 s of the reactions catalyzed by the flavoprotein N-acetylpolyamine oxidase (PAO) and the pterin-dependent enzymes phenylalanine hydroxylase (PheH) and tyrosine hydroxylase (TyrH). N, N′-Dibenzyl-1, 4-diaminobutane (DBDB) is a substrate for PAO for which amine oxidation is rate-limiting. CFMS of the reaction showed formation of ...