Tetrahedron

Causes of unreproducibility of C. rugosa lipase-catalyzed reactions in slightly hydrated organic media

PD de María, JVS Gago

文献索引：Dominguez De Maria, Pablo; Sinisterra Gago, Jose V. Tetrahedron, 1999 , vol. 55, # 28 p. 8555 - 8566

全文：HTML全文

被引用次数: 20

摘要

Lipase activity, measured as hydrolysis of tributyrin is a valid assay to quantify the lipase activity of a lyophilized crude lipase in hydrolysis reactions but it is not useful to predict the catalytic activity in lipase-catalyzed reactions in organic media. Three factors control the catalytic activity in these media: i) relative proportion of isoenzymes; ii) amount of water in the lyophilized crude enzyme and iii) amount of lipase protein in the commercial powder. ...