Oriented immobilization of antibodies with GST-fused multiple Fc-specific B-domains on a gold surface

…, JM Lee, KY Lee, Y Lee, JS Park, BH Chung

文献索引：Ha, Tai Hwan; Jung, Sun Ok; Lee, Jeong Min; Lee, Kun Yeong; Lee, Yan; Park, Jong Sang; Chung, Bong Hyun Analytical Chemistry, 2007 , vol. 79, # 2 p. 546 - 556

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被引用次数: 61

摘要

We have constructed a novel platform for the oriented buildup of immunoglobulins on a gold surface for a surface plasmon resonance imaging microarray. To this end, genetically engineered glutathione S-transferase proteins bearing one, two, and three Fc-specific B- domains in protein G from Streptococci (GST-GB1,-GB2, and-GB3, respectively) were produced. In order to tether these GST-GB x proteins specifically, a novel glutathione- ...