The heme-thiolate proteins comprise a large number of important enzymes such as cytochrome P450,['] NO-synthase, l2] and chloroperoxidase (CPO). r31 The inherent reactivity of these enzymes is attributed to an iron protoporphyrin IX complex located at the active center. It is connected to the protein by hydrogen bridges of the two propionate side chains, hydrophobic interactions, and a thiolate ligand coordinated to the iron. The thiolate ...
