Figure 3. Nonprotonated aromatic carbon spectra of fd coat protein.(a) Isolatcd solid fd coat protein; cross polarized with I-ms mix time, 3-s recycle delay, 0.1-s acquisition time, and a 90+ s delay without decoupling prior to data acquisition for 20 000 transients.(b) Solid fd virus: Same conditions as (a).(c) Detergent solubilized fd coat protein in water: sample ih 6 niM in protein (IO mL) in 20-mm tube; weak noise-modulated proton decoupling is applied ...
