Bioorganic & medicinal chemistry letters

Dual isotope labeling: conjugation of 32 P-oligonucleotides with 18 F-aryltrifluoroborate via copper (I) catalyzed cycloaddition

Y Li, P Schaffer, DM Perrin

文献索引：Li, Ying; Schaffer, Paul; Perrin, David M. Bioorganic and Medicinal Chemistry Letters, 2013 , vol. 23, # 23 p. 6313 - 6316

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被引用次数: 7

摘要

Abstract A one-pot-two-step labeling of an oligonucleotide with an 18 F-ArBF 3−(aryltrifluoroborate) radioprosthetic is reported herein. In order to characterize labeling in terms of radiochemistry, phosphorus-32 was also introduced to the 5′-terminus of the oligonucleotide via enzymatic phosphorylation. A pendant azide group was subsequently conjugated to the 5′-phosphate of the oligonucleotide. Copper (I) catalyzed [2+ 3] ...