The Use of Biochemical and Biophysical Tools for Triage of High??Throughput Screening Hits–A Case Study with Escherichia coli Phosphopantetheine …

…, M Lall, C Donovan, RW Sarver, DY Lee…

文献索引：Miller, J. Richard; Thanabal, Venkataraman; Melnick, Michael M.; Lall, Manjinder; Donovan, Charles; Sarver, Ronald W.; Lee, Doh-Yeel; Ohren, Jeff; Emerson, Don Chemical Biology and Drug Design, 2010 , vol. 75, # 5 p. 444 - 454

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被引用次数: 19

摘要

High-throughput screening is utilized by pharmaceutical researchers and, increasingly, academic investigators to identify agents that act upon enzymes, receptors, and cellular processes. Screening hits include molecules that specifically bind the target and a greater number of non-specific compounds. It is necessary to'triage'these hits to identify the subset worthy of further exploration. As part of our antibacterial drug discovery effort, we applied a ...