Bioorganic & medicinal chemistry

New cyanine dyes as base surrogates in PNA: forced intercalation probes (FIT-probes) for homogeneous SNP detection

L Bethge, DV Jarikote, O Seitz

文献索引：Bethge, Lucas; Jarikote, Dilip Venkatrao; Seitz, Oliver Bioorganic and Medicinal Chemistry, 2008 , vol. 16, # 1 p. 114 - 125

全文：HTML全文

被引用次数: 75

摘要

Forced intercalation probes (FIT-probes) are nucleic acid probes, in which an intercalator cyanine dye such as thiazole orange (TO) serves as a replacement of a canonical nucleobase. These probes signal hybridization by showing strong increases of fluorescence. TO in FIT-probes responds to adjacent base mismatches by attenuation of fluorescence intensities at conditions where both matched and mismatched target DNA ...