Elucidation of the coordination chemistry of the enzyme-substrate complex of catechol 1, 2-dioxygenase by NMR spectroscopy

…, RB Lauffer, JB Lynch, BP Murch…

文献索引：Que, Lawrence; Lauffer, Randall B.; Lynch, John B.; Murch, Bruce P.; Pyrz, Joseph, W. Journal of the American Chemical Society, 1987 , vol. 109, # 18 p. 5381 - 5385

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被引用次数: 36

摘要

Abstract: The coordination chemistry of the active site iron in catechol 1, 2-dioxyygenase (CTD) from Pseudomonas putida (ATCC 23974) has been probed with NMR spectroscopy. The high-spin ferric center gives rise to paramagnetically shifted resonances in the range of 105 to-67 ppm, assigned to protons on the iron ligands. Observed endogenous ligand resonances are assigned to P-CH2 protons of tyrosines and histidines. Exogenous ligand ...