Enantioselective acylation of β-phenylalanine acid and its derivatives catalyzed by penicillin G acylase from Alcaligenes faecalis

D Li, L Ji, X Wang, D Wei

文献索引：Li, Dengchao; Ji, Lilian; Wang, Xinfeng; Wei, Dongzhi Preparative Biochemistry and Biotechnology, 2013 , vol. 43, # 2 p. 207 - 216

全文：HTML全文

被引用次数: 2

摘要

This study developed a simple, efficient method for producing racemic β-phenylalanine acid (BPA) and its derivatives via the enantioselective acylation catalyzed by the penicillin G acylase from Alcaligenes faecalis (Af-PGA). When the reaction was run at 25° C and pH 10 in an aqueous medium containing phenylacetamide and BPA in a molar ratio of 2: 1, 8 U/mL enzyme and 0.1 M BPA, the maximum BPA conversion efficiency at 40 min only reached ...