The final step in the degradation of heparin sulfate involves the enzymatic hydrolysis of its 2- sulfamido groups. To evaluate the power of the corresponding sulfamidases as catalysts, we examined the reaction of N-neopentyl sulfamate at elevated temperatures and found it to undergo specific acid catalyzed hydrolysis even at alkaline pH. A rate constant of 10–16 s–1 was calculated using the Eyring equation for water attack on the N-protonated species at ...
