Reduction mechanism of tetrazolium salt XTT by a glucosamine derivative

…, A Takamori, H Ukeda, M SAWAMURA

文献索引：Shimamura, Tomoko; Takamori, Atsuko; Ukeda, Hiroyuki; Sawamura, Masayoshi Bioscience, Biotechnology and Biochemistry, 2003 , vol. 67, # 2 p. 295 - 299

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被引用次数: 9

摘要

XTT (3′-{1-[(phenylamino)-carbonyl]-3, 4-tetrazolium}-bis (methoxy-6-nitro) benzenesulfonic acid hydrate) was reduced by incubated glucosamine hydrochloride. The XTT reducibility by incubated glucosamine was linearly related with the DNA-breaking activity. In order to elucidate the reaction mechanism, the glucosamine derivatives formed during the incubation process were separated by HPLC, and the compound responsible ...