Organic & biomolecular chemistry

γ-Glutamyl transpeptidase acylation with peptidic substrates: free energy relationships measured by an HPLC kinetic assay

M Morin, C Rivard, JW Keillor

文献索引：Morin, Mylene; Rivard, Caroline; Keillor, Jeffrey W. Organic and Biomolecular Chemistry, 2006 , vol. 4, # 20 p. 3790 - 3801

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被引用次数: 12

摘要

γ-Glutamyl transpeptidase (GGT, EC 2.3. 2.2) is a highly glycosylated heterodimeric enzyme linked to the external cellular membrane that catalyzes the hydrolysis of glutathione as well as the transfer of its γ-glutamyl group to amino acids and dipeptides in a transpeptidation reaction. The measurement of both the hydrolytic and transpeptidation activity of this important enzyme is a challenge, since its native substrates are not highly chromogenic. ...