Biosynthesis of 4-Methylproline in Cyanobacteria: Cloning of n osE and n osF Genes and Biochemical Characterization of the Encoded Dehydrogenase and …
H Luesch, D Hoffmann, JM Hevel…
文献索引：Luesch, Hendrik; Hoffmann, Dietmar; Hevel, Joan M.; Becker, Julia E.; Golakoti, Trimurtulu; Moore, Richard E. Journal of Organic Chemistry, 2003 , vol. 68, # 1 p. 83 - 91
The biosynthesis of the unusual amino acid 4-methylproline in the Nostoc genus of cyanobacteria was investigated on the genetic and enzymatic level. Two genes involved in the biosynthesis were cloned and the corresponding enzymes, a zinc-dependent long-chain dehydrogenase and a Δ1-pyrroline-5-carboxylic acid (P5C) reductase homologue, were overexpressed in Escherichia coli and biochemically characterized. Putative substrates ...
