Preparation of D-amino acids by enzymatic kinetic resolution using a mutant of penicillin-G acylase from E. coli
C Carboni, HGT Kierkels, L Gardossi, K Tamiola…
文献索引：Carboni, Chiara; Kierkels, Hans G. T.; Gardossi, Lucia; Tamiola, Kamil; Janssen, Dick B.; Quaedflieg, Peter J. L. M. Tetrahedron Asymmetry, 2006 , vol. 17, # 2 p. 245 - 251
We have demonstrated for the first time that d-glutamine (d-Gln) and d-glutamic acid (d-Glu) can be efficiently obtained in high ee (97% and 90%, respectively) by enzymatic kinetic resolution of d, l-Gln and d, l-Glu. This was achieved by enantioselective conversion of the l- enantiomers to their N-phenylacetyl derivatives in aqueous solution, using a mutant of penicillin-G acylase (PGA) from E. coli and phenylacetic acid methylester as the acyl ...
