Maltotetraose-forming, amylase-mediated, p-nitrophenyl α-and β-maltopentaoside formation in an aqueous—organic solvent system: a substrate for human amylase …
In recent years, malto-oligosaccharide derivatives of defined structure have been used in clinical laboratories'” for the measu rement of alpha-amylase activity in human serum and urine. One such chromogenic substrate2.'is pNP-aG,. However, conventional methods for obtaining pNP-aG,, either chemically or enzymically, give only low yields of the desired compound 8q9 We have reported that G,-amylase from Pseudomo-. nas stutzeri forms ...
