Organic co-solvents restore the inherently high enantiomeric ratio of lipase B from Candida antarctica in hydrolytic resolution by relieving the enantiospecific inhibition …
The enantiomeric ratio in the hydrolysis of racemic 3-chloro-1-phenylmethoxy-2-propyl butanoate with lipase B from Candida antarctica, CALB, is raised from E= 50 to E= 180 upon addition of acetone to the aqueous medium. This co-solvent effect is now explained as an enantiospecific inhibition of the lipase by the liberated alcohol. In the range from 0 to 30% acetone, the effect correlates with the increased solubility of the alcohol in the reaction ...
