L-Lactate dehydrogenase: substrate specificity and use as a catalyst in the synthesis of homochiral 2-hydroxy acids

MJ Kim, GM Whitesides

文献索引：Kim,M.J.; Whitesides,G.M. Journal of the American Chemical Society, 1988 , vol. 110, p. 2959

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被引用次数: 129

摘要

Abstract: This paper reports kinetic parameters (Km, kat) for reduction of approximately 20 a- keto acids by L-lactate dehydrogenase (L-LDH; EC 1.1. 1.27) from 5 sources (porcine heart, rabbit muscle, chicken liver, bovine heart, lobster tail). The~-LDH-catalyzed reduction reaction of four substrates representative of the range of activities observed has been carried out on a preparative scale using rabbit muscle L-LDH, and absolute configurations ...