A fluorescent sensor for GABA and synthetic GABAB receptor ligands

A Masharina, L Reymond, D Maurel…

文献索引：Brun, Matthias A.; Tan, Kui-Thong; Griss, Rudolf; Kielkowska, Anna; Reymond, Luc; Johnsson, Kai Journal of the American Chemical Society, 2012 , vol. 134, # 18 p. 7676 - 7678

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被引用次数: 36

摘要

While γ-aminobutyric acid (GABA) is the main inhibitory neurotransmitter, suitable tools to measure its concentration in living cells with high spatiotemporal resolution are missing. Herein, we describe the first ratiometric fluorescent sensor for GABA, dubbed GABA-Snifit, which senses GABA with high specificity and spatiotemporal resolution on the surface of living mammalian cells. GABA-Snifit is a semisynthetic fusion protein containing the ...