The Journal of organic chemistry

Discovery of a sensitive, selective, and tightly binding fluorogenic substrate of bovine plasma amine oxidase

KQ Ling, LM Sayre

文献索引：Ling, Ke-Qing; Sayre, Lawrence M. Journal of Organic Chemistry, 2009 , vol. 74, # 1 p. 339 - 350

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被引用次数: 10

摘要

We report a novel fluorogenic substrate of bovine plasma amine oxidase (BPAO), namely,(2- (6-(aminomethyl) naphthalen-2-yloxy) ethyl) trimethylammonium (ANETA), which displays extremely tight binding to BPAO (K m 183±14 nM) and yet is metabolized fairly quickly (k cat 0.690±0.010 s− 1), with the aldehyde turnover product (2-(6-formylnaphthalen-2-yloxy) ethyl) trimethylammonium serving as a real time reporting fluorophore of the enzyme ...