Discovery and characterization of a thermostable d-lactate dehydrogenase from Lactobacillus jensenii through genome mining
…, YS Sa, SA Gu, JC Joo, S Kim, KJ Kim, YH Kim
文献索引:Jun, Chanha; Sa, Young Seung; Gu, Sol-A; Joo, Jeong Chan; Kim, Seil; Kim, Kyung-Jin; Kim, Yong Hwan Process Biochemistry, 2013 , vol. 48, # 1 p. 109 - 117
The demand on thermostable d-lactate dehydrogenases (d-LDH) has been increased for d- lactic acid production but thermostable d-DLHs with industrially applicable activity were not much explored. To identify a thermostable d-LDH, three d-LDHs from different Lactobacillus jensenii strains were screened by genome mining and then expressed in Escherichia coli. One of the three d-LDHs (d-LDH3) exhibited higher optimal reaction temperature (50° C) ...