Chemically modified “polar patch” mutants of subtilisin in peptide synthesis with remarkably broad substrate acceptance: designing combinatorial biocatalysts

K Matsumoto, BG Davis…

文献索引：Matsumoto, Kazutsugu; Davis, Benjamin G.; Jones, J. Bryan Chemistry - A European Journal, 2002 , vol. 8, # 18 p. 4129 - 4137

全文：HTML全文

被引用次数: 29

摘要

Abstract A significant enhancement of the applicability of the serine protease subtilisin Bacillus lentus (SBL) in peptide synthesis was achieved by using the strategy of combined site-directed mutagenesis and chemical modification to create chemically modified mutant (CMM) enzymes. The introduction of polar and/or homochiral auxiliary substituents, such as X= oxazolidinones, alkylammonium groups, and carbohydrates at position 166 at the base ...