Industrial production of chiral intermediate of cilastatin by nitrile hydratase and amidase catalyzed one-pot, two-step biotransformation

RC Zheng, ZY Yang, CC Li, YG Zheng…

文献索引：Zheng, Ren-Chao; Yang, Zhong-Yi; Li, Cong-Cheng; Zheng, Yu-Guo; Shen, Yin-Chu Journal of Molecular Catalysis B: Enzymatic, 2014 , vol. 102, p. 161 - 166

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被引用次数: 4

摘要

Abstract An industrial one-pot, two-step bioprocess catalyzed by nitrile hydratase (NHase) and amidase was developed for (S)-2, 2-dimethylcyclopropanecarboxamide (1), the key chiral intermediate of cilastatin. The key unit operations of the whole process including fermentative production of enzymes, biotransformation, isolation of product, and recycling of by-product were reported for the first time. The volumetric enzyme activities of NHase and ...