... by incubating 300 μl of the membrane preparation with six to eight increasing concentrations of [ 3 H]DHA (1.1 to 11.0 nM in most cases) in the presence or absence of 10 –3 M L-isoproterenol, in a total volume of 450 μl. ... 2. ... 1963; 34:320–4. CrossRef | Web of Science | Medline. 9. ...