Solid-phase oligodeoxynucleotide synthesis: a two-step cycle using peroxy anion deprotection
…, DJ Dellinger, JR Betley, TK Wyrzykiewicz…
文献索引：Sierzchala, Agnieszka B.; Dellinger, Douglas J.; Betley, Jason R.; Wyrzykiewicz, Tadeusz K.; Yamada, Christina M.; Caruthers, Marvin H. Journal of the American Chemical Society, 2003 , vol. 125, # 44 p. 13427 - 13441
A novel solid-phase phosphoramidite based oligodeoxynucleotide two-step synthesis method has been developed. Keys to this method are replacement of the 5'-dimethoxytrityl blocking group with an aryloxycarbonyl and the use of N-dimethoxytrityl protection for the exocyclic amines of adenine and cytosine. With these modifications, coupling of each 2'- deoxynucleoside 3'-phosphoramidite to the growing oligodeoxynucleotide on the solid ...
