Remarkable Effect of D-Sorbitol on the Second Stage in the PLE-Catalyzed Hydrolyses of a. SIGMA.-Symmetric Diester, cis-Cyclohex-4-ene-1, 2-bis (methyl acetate).
Enantioselective hydrolysis of cis-cyclohex-4-ene-1, 2-bis (methyl acetate) with porcine liver esterase (PLE) in the presence of D-sorbitol afforded a chiral monoester in a highly enantioselective manner. During the enzymatic hydrolyses, D-sorbitol efficiently accelerate the second stage; hydrolysis of the resultant minor monoester toward the dicarboxylic acid leaving the desired major monoester.
