... Il Farmaco. Volume 58, Issue 9, September 2003, Pages 929–937. ... 100 mmol NaCl, 10 mmol EDTA, 0.5% NP 4 O, 0.5% NaDOC, 10 mmol Tris–HCl pH 4) and centrifuged at 6,700 rpm for 15 min at 4 °C. The supernatant was supplemented with an equal volume of sarcosyl 20 ...