Abstract A method for solid-phase detection of phospholipase A 2 (PLA 2) was developed. The method uses 1-octanoyloxynaphthalene-3-sulfonic acid, which was found to be a good substrate of PLA 2. The substrate is hydrolyzed by PLA 2 into 1-naphthol-3-sulfonic acid, which is spontaneously coupled with coexisting diazonium salt to form a red-purple azo dye. Streptomyces and bovine pancreatic PLA 2 spotted on a nitrocellulose membrane could ...
