Further optimization of detritylation in solid-phase oligodeoxyribonucleotide synthesis

K Tram, YS Sanghvi, H Yan

文献索引：Tram, Kha; Sanghvi, Yogesh S.; Yan, Hongbin Nucleosides, Nucleotides and Nucleic Acids, 2011 , vol. 30, # 1 p. 12 - 19

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被引用次数: 6

摘要

Various conditions for optimum detritylation (ie, the removal of 5′-O-trityl protecting groups) during solid-phase synthesis of oligodeoxyribonucleotides were investigated. Di-and tri- chloroacetic acids of variable concentrations were used to study the removal of the 4, 4′- dimethoxytrityl (DMTr) group. It was found that the DMTr group could be completely removed under much milder acidic conditions than what are currently used for automated solid- ...