Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation

ET Farinas, U Schwaneberg…

文献索引：Farinas, Edgardo T.; Schwaneberg, Ulrich; Glieder, Anton; Arnold, Frances H. Advanced Synthesis and Catalysis, 2001 , vol. 343, # 6-7 p. 601 - 606

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被引用次数: 153

摘要

Abstract: Cytochrome P450 monooxygenase BM-3 (EC 1.14. 14.1) hydroxylates fatty acids with chain lengths between C12 and C18. It is also known to oxidize the corresponding alcohols and amides. However, it is not known to oxidize alkanes. Here we report that P450 BM-3 oxidizes octane, which is four carbons shorter and lacks the carboxylate functionality of the shortest fatty acid P450 BM-3 is known to accept, to 4-octanol, 3-octanol, 2-octanol, ...