Immobilization of P450 BM-3 monooxygenase on mesoporous molecular sieves with different pore diameters

…, T Schreiber, B Thomas, J Pleiss, M Hunger…

文献索引：Weber, Evelyne; Sirim, Demet; Schreiber, Tino; Thomas, Bejoy; Pleiss, Juergen; Hunger, Michael; Glaeser, Roger; Urlacher, Vlada B. Journal of Molecular Catalysis B: Enzymatic, 2010 , vol. 64, # 1-2 p. 29 - 37

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被引用次数: 38

摘要

The immobilization of the isolated heme domain of P450 BM-3 (BM3H_F87A) on two mesoporous molecular sieves, MCM-41 (pore diameter 25Å) and SBA-15 (pore diameter 60Å and 133Å) was examined systematically, and the activity of the immobilized enzyme toward para-nitrophenoxydodecanoic acid (12-pNCA) and n-octane was determined. Hydrogen peroxide was utilized as source of electrons and oxygen to support the ...