Liebigs Annalen der Chemie

Preparative??enzymatic formation of cytidine 5′??monophosphosialate by integrated cytidine 5′??triphosphate regeneration

J Thiem, P Stangier

文献索引：Thiem, Joachim; Stangier, Peter Liebigs Annalen der Chemie, 1990 , # 11 p. 1101 - 1105

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被引用次数: 15

摘要

Abstract The preparative formation of cytidine 5′-triphosphate by an enzymatic process with adenylate kinase and pyruvate kinase is described. The enzymes may either be used free, in the “MEEC” technique, or immobilized on a synthetic copolymer of vinyl acetate and N, N′-divinylethyleneurea (“VA-Epoxy”). Cytidine 5′-monophosphosialate synthase (preferably isolated from calf brain) and inorganic pyrophosphatase were used to prepare ...