Biochemistry

NMR-derived models of amidopyrine and its metabolites in complexes with rabbit cytochrome P450 2B4 reveal a structural mechanism of sequential N-dealkylation

…, SEA Sjögren, N Fomina, KT Vu, A Almutairi…

文献索引：Roberts, Arthur G.; Sjoegren, Sara E. A.; Fomina, Nadezda; Vu, Kathy T.; Almutairi, Adah; Halpert, James R. Biochemistry, 2011 , vol. 50, # 12 p. 2123 - 2134

全文：HTML全文

被引用次数: 11

摘要

To understand the molecular basis of sequential N-dealkylation by cytochrome P450 2B enzymes, we studied the binding of amidopyrine (AP) as well as the metabolites of this reaction, desmethylamidopyrine (DMAP) and aminoantipyrine (AAP), using the X-ray crystal structure of rabbit P450 2B4 and two nuclear magnetic resonance (NMR) techniques: saturation transfer difference (STD) spectroscopy and longitudinal (T 1) relaxation NMR. ...