Synthesis of pyrophosphates for in vitro selection of catalytic RNA molecules

HJ Kim, MJ Kim, N Karalkar, D Hutter…

文献索引：Kim, Hyo-Joong; Kim, Myong Jung; Karalkar, Nilesh; Hutter, Daniel; Benner, Steven A. Nucleosides, Nucleotides and Nucleic Acids, 2008 , vol. 27, # 1 p. 43 - 56

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被引用次数: 6

摘要

Reported here are synthetic routes to pyrophosphates linking riboflavin with various nucleosides. The focus is on a flavin-uracil dinucleotide having a biotin tag on the uracil, a molecule that has potential value in the selection of RNA enzymes that catalyze the template- directed polymerization of RNA in the 3′-to-5′ direction, which is the direction opposite that catalyzed by standard protein polymerases. Two detailed procedures are presented ...