Pivalase catalytic antibodies: towards abzymatic activation of prodrugs

…, MT Reymond, JL Reymond

文献索引：Bensel, Nicolas; Reymond, Martine T.; Reymond, Jean-Louis Chemistry - A European Journal, 2001 , vol. 7, # 21 p. 4604 - 4612

全文：HTML全文

被引用次数: 27

摘要

Abstract Screening of monoclonal-antibody libraries generated against the tert-butyl phosphonate hapten 2 and the chloromethyl phosphonate hapten 3 with pivaloyloxymethyl- umbelliferone 1 as a fluorogenic substrate led to the isolation of eleven catalytic antibodies with rate accelerations around k cat/k uncat= 10 3. The antibodies are not inhibited by the product and accept different acyloxymethyl derivatives of acidic phenols as substrates. ...